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EMS0011

Sigma-Aldrich

Low-Artifact Digestion Buffer

Minimizes artifactual deamidation and oxidation during trypsin digestion prior to peptide mapping

Sinonimo/i:

Hi-Fidelity Peptide Mapping Buffer

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About This Item

Codice UNSPSC:
41105331
NACRES:
NA.25

Stato

liquid

Compatibilità

suitable for (peptide mapping)

Condizioni di spedizione

wet ice

Temperatura di conservazione

2-8°C

Descrizione generale

Low-Artifact Digestion Buffer (EMS0011) is optimized for digestion of therapeutic proteins and antibodies prior to peptide mapping by LC-UV-MS. The buffer has been designed to minimize artifactual methionine oxidation and asparagine deamidation during sample preparation while maintaining effective reduction, alkylation and proteolytic digestion.

Endogenous deamidation and oxidation of protein amino acids can be implicated in and be indicative of many diseases, protein turnover, development, and aging. The introduction of these modifications can often affect biological activity, half-life, and immunogenicity.

Current protein digestion workflows often introduce a significant amount of artifactual deamidation and oxidation which prohibits the measurement of the endogenous levels.

The Low Artifact Digestion Buffer allows protein digestion in less than 6 hours with minimized artifactual modifications such as deamidation and oxidation. The amount of oxidation and deamidation introduced during further downstream processing of biotherapeutic antibodies and proteins can be accurately determined. The most commonly deamidated amino acid is Asn especially when followed by small amino acids such as Gly. The most commonly oxidized amino acids are Met, Cys, and to some extent Trp and His.

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

nwg

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


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Articoli

Step-by-step workflows for the intact mass analysis, peptide mapping, and N-glycan analysis of the monoclonal antibody― adalimumab, for an accurate characterization of the critical quality attributes (CQAs) to ensure drug safety and efficacy. Read more.

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