E8008

Ethylenediaminetetraacetic acid solution

0.02% in DPBS (0.5 mM), sterile-filtered, BioReagent, suitable for cell culture

• Sinonimo/i: EDTA solution
• Formula empirica (notazione di Hill): C₁₀H₁₆N₂O₈
• Numero CAS: 60-00-4
• Peso molecolare: 292.24
• Beilstein: 1716295
• Numero MDL: MFCD00003541
• Codice UNSPSC: 12352204
• eCl@ss: 39030907
• ID PubChem: 24894664
• NACRES: NA.75

Proprietà

• Sterilità: sterile-filtered
• Livello qualitativo: 200
• Nome Commerciale: BioReagent
• Stato: solution
• Concentrazione: 0.02% in DPBS (0.5 mM)
• tecniche: cell culture | mammalian: suitable
• Impurezze: endotoxin, tested
• pH: 7.1-7.7
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: 2-8°C
• Stringa SMILE: OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
• InChI: 1S/C10H16N2O8/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20); KCXVZYZYPLLWCC-UHFFFAOYSA-N

Descrizione

Descrizione generale

Ethylenediaminetetraacetic acid (EDTA) is an aminopolycarboxylic acid and a hexadentate ligand. It chelates with metal ions to form an octahedral complex especially with cations. Ethylenediaminetetraacetic acid (EDTA) is an anticoagulant, which prevents clotting of blood. It is implicated in platelet aggregation in pseudothrombocytopenia. EDTA chelates with calcium and is effective in solubilisation of the atheromatous plaques. Calcium disodium EDTA administration is effective in slowing down the progression of chronic kidney disease. It is a chelator used to remove calcium from cell washing and suspension media. EDTA reduces cell clumping.

Applicazioni

Ethylenediaminetetraacetic acid solution has been used to remove confluent cell monolayers in swine testicular (ST-cells) culture. It has also been used to detach embryonic stem cells (ESCs) from matrigel and primary human fibroblasts.

Stato fisico

Solution in Dulbecco′s phosphate buffered saline without calcium and magnesium

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): nwg
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable