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E6406

Millipore

EZview™ Red Glutathione Affinity Gel

Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
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About This Item

Numero MDL:
MFCD05665380
Codice UNSPSC:
41106500
NACRES:
NA.56

Classi chimiche degli analiti

proteins (GST)

tecniche

immunoprecipitation (IP): suitable
protein purification: suitable

Temperatura di conservazione

2-8°C

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Categorie correlate

Descrizione generale

EZviewä Red Glutathione Affinity Gel is a highly visible, red colored glutathione agarose affinity gel. The affinity resin is composed of glutathione covalently attached through the sulfur to epoxy-activated 4% cross-linked agarose beads, resulting in a 12-atom spacer.

Applicazioni

The product is used for affinity capture, molecular pull-down, or IP of proteins containing glutathione binding sequences, such as glutathione S-transferase, glutathione peroxidase and glyoxalase I.
When performing small-scale affinity capture, such as immunoprecipitation, the affinity matrix is difficult to see in the microcentrifuge tubes. Accidental aspiration of the resin leads to quantitative variability in results. The EZview™ Red Affinity Gels greatly reduce the risk of pellet loss. EZview™ resins perform as well as conventional non-colored affinity gels, but allow the user to easily differentiate pellet from supernatant. This correlates to more accurate data because less protein is lost.

Caratteristiche e vantaggi

  • Increased visibility - Red color reduces risk of incidental aspiration
  • Improved recovery of target protein by reduced accidental loss
  • Higher reproducibility - More consistent yields

Stato fisico

1:1 (v/v) suspension in PBS containing 50% glycerol and 15 ppm Kathon

Note legali

EZview is a trademark of Sigma-Aldrich Co. LLC

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Certificati d'analisi (COA)

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S K Jang et al.
Journal of virology, 62(8), 2636-2643 (1988-08-01)
Picornavirus RNAs are uncapped messengers and have unusually long 5' nontranslated regions (5'NTRs) which contain many noninitiating AUG triplets. The translational efficiency of different picornavirus RNAs varies between different cell-free extracts and even in the same extract, such as micrococcal
P L Hallauer et al.
BMC genetics, 1, 1-1 (2000-10-19)
Versatile transgenic manipulation of skeletal muscle requires knowledge of the expression profiles of diverse promoter/enhancer elements in the transcriptionally specialized fiber types of which muscle is composed. "Universal" viral promoters/enhancers, e.g., cytomegalovirus IE1 (CMV IE1), are of interest as reagents
S Andersson et al.
The Journal of biological chemistry, 264(14), 8222-8229 (1989-05-15)
The conversion of cholesterol into bile acids in the liver represents the major catabolic pathway for the removal of cholesterol from the body. In this complex biosynthetic pathway, at least 10 enzymes modify both the ring structure and side chain
D R Thomsen et al.
Proceedings of the National Academy of Sciences of the United States of America, 81(3), 659-663 (1984-02-01)
The DNA templates containing immediate early (IE) genes of human cytomegalovirus (CMV) were transcribed in vitro by using a HeLa cell extract. When IE region 1, 2, and 3 were used, transcription was detected qualitatively only from IE region 1.

Contenuto correlato

Protein Pull-Down

Pull-down assays, reagents, and protocols for investigating in vitro protein-protein interactions using affinity or GST pull-down, tandem affinity purification (TAP), and co-immunoprecipitation methods.

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