E2884
Esterase from porcine liver
ammonium sulfate suspension, ≥150 units/mg protein (biuret)
Sinonimo/i:
Carboxyl esterase, Carboxylic-ester hydrolase, PLE
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About This Item
Numero CAS:
Numero MDL:
Codice UNSPSC:
12352204
NACRES:
NA.54
Prodotti consigliati
Origine biologica
Porcine liver
Forma fisica
ammonium sulfate suspension
Attività specifica
≥150 units/mg protein (biuret)
PM
168 kDa
Temperatura di conservazione
2-8°C
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Descrizione generale
Esterase, also known as carboxyl ester hydrolase is a member of the hydrolase superfamily of enzymes.
Applicazioni
Esterase from porcine liver has been used to unmask the fluorescence in ultraviolet-visible spectroscopy.
The enzyme from Sigma has been used to develop an alternative gel-based protocol for screening for bead-bound catalytic activity in aqueous media. The product has also been used to investigate the properties of the compound L-valine-3-{8-[(E)-2-[3-methoxyphenyl)ethenyl]-7-methyl-1-propargylxanthine-3-yl}propyl ester hydrochloride (MSX-4, an aminoacid ester prodrug of the adenosine A2A receptor antagonist MSX-2).
Porcine liver esterase is used to catalyze the hydrolysis of pentaacetyl catechin and epicatechin for use in pharmaceutical and industrial applications.
Pig liver esterase is commonly used for kinetic resolutions and assymetric synthesis in organic chemistry.
Pig liver esterase is commonly used for kinetic resolutions and assymetric synthesis in organic chemistry.
Azioni biochim/fisiol
Esterase acts on water-soluble carboxyl esters containing short chain fatty acids. Its functionality is attributed to the catalytic triad of Ser, His and Asp/Glu.
Esterase enzyme of bacteria and fungi seems to have several industrial applications. It is capable of catalyzing the hydrolysis and synthesis of ester bonds.
Pig liver esterase catalyzes enantioselective conversion of an ester to a carboxylic acid. The molecular weight is found to be 168 kDa. It is a serine enzyme with two active sites on each molecule, which dissociates into active half-molecules in the presence of dilute acid or concentrated salts.
Definizione di unità
One unit will hydrolyze 1.0 μmole of ethyl butyrate to butyric acid and ethanol per min at pH 8.0 at 25 °C.
Stato fisico
Suspension in 3.2 M (NH4)2SO4, pH 8
Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Certificati d'analisi (COA)
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Articoli
Instructions for working with enzymes supplied as ammonium sulfate suspensions
Protocolli
Enzymatic Assay of Esterase
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