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DMN70

Sigma-Aldrich

GenElute™ Direct mRNA Miniprep Kits

sufficient for 70 purifications

Sinonimo/i:

GenElute™ Direct mRNA Miniprep Kit, GenElute™ mRNA Kit, Gen Elute

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About This Item

Codice UNSPSC:
41105501
NACRES:
NA.52

impiego

sufficient for 70 purifications

Livello qualitativo

200

Temperatura di conservazione

15-25°C

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Descrizione generale

The GenElute™ Direct mRNA Miniprep kit provides a convenient format to isolate polyadenylated mRNA directly from mammalian cells and tissues. The direct mRNA isolation procedure is based on that of Badley. Up to 107 mammalian cells or 40 mg tissue are lysed and homogenized, either with the filtration columns provided or with
a mechanical homogenizer. RNase is eliminated during a 10 minute proteinase K digestion. Sodium chloride is added, and polyadenylated RNA is captured on oligo(dT) polystyrene beads during a 10 minute incubation. For further enrichment, RNA may be released from the beads into fresh lysis solution and recaptured with the original beads. After 3 washes in a spin column, purified mRNA is eluted in 100 μl of 10 mM Tris-HCl, pH 7.4.

Applicazioni

GenElute™ Direct mRNA Miniprep Kit has been used to isolate mRNA from various tissues and cells.
The GenElute Direct Kit mRNA kit provides convenient procedures for isolating polyadenylated mRNA from previously prepared total RNA or directly from mammalian cells and tissues.

Caratteristiche e vantaggi

  • mRNA captured on oligo(dT) polystyrene beads in 10 minutes, with no mixing or rocking (Fig. 1)
  • Poly (A)+ mRNA isolated from total RNA in 40 minutes (Fig. 2) or 60 minutes directly from cells and tissues (Fig. 3)
  • Oligo(dT) polystyrene beads require fewer wash steps

Altre note

Use for isolating mRNA directly from mammalian cells or tissues.
For additional information, please see www.sigma-aldrich.com/mrna.

Note legali

GenElute is a trademark of Sigma-Aldrich Co. LLC

Solo come componenti del kit

N° Catalogo
Descrizione
  • Collection tube 70 ea
  • Elution solution 10 mL
  • Filtration columns with tubes 70 ea
  • Lysis solution 120 mL
  • 5 M NaCl 8 mL
  • Oligo(dT)-polystyrene beads 2 mL
  • Proteinase K 25 mg
  • Spin columns with tubes 70 ea
  • 40% Glycerol solution 3 mL
Vedi tutto (9)

Avvertenze

Danger

Indicazioni di pericolo

H290,H315,H319,H334,H335

Classi di pericolo

Eye Irrit. 2 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Organi bersaglio

Respiratory system

Codice della classe di stoccaggio

8A - Combustible corrosive hazardous materials

Certificati d'analisi (COA)

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Inanc B
Tissue Engineering: Part A, 14(6), 955-964 (2008)

Articoli

Genomic DNA Sample Purification and Quality Assessment

The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RNA rapidly and conveniently from a variety of cellular sources, including cells and tissues from mammalian, plant and bacterial cultures.

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

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