D2693
Anti-phospho-DARPP32 (pThr75) antibody produced in rabbit
affinity isolated antibody, buffered aqueous glycerol solution, sufficient for 10 blots
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About This Item
Prodotti consigliati
Origine biologica
rabbit
Livello qualitativo
Coniugato
unconjugated
Forma dell’anticorpo
affinity isolated antibody
Tipo di anticorpo
primary antibodies
Clone
polyclonal
Forma fisica
buffered aqueous glycerol solution
impiego
sufficient for 10 blots
PM
antigen 32 kDa
Reattività contro le specie
mouse, human
tecniche
dot blot: 1:1,000
western blot: 1:1,000
N° accesso UniProt
Condizioni di spedizione
wet ice
Temperatura di conservazione
−20°C
Informazioni sul gene
human ... PPP1R1B(84152)
mouse ... Ppp1r1b(19049)
Descrizione generale
DARPP32 is a phosphoprotein with a molecular mass of 32kDa. It is regulated by dopamine (DA) and cAMP and is associated with dopaminoceptive neurons bearing D-1 receptors in the basal ganglia. DARPP32 is a target for the actions of dopamine.
Immunogeno
synthetic phosphopeptide corresponding to amino acids residues surrounding the DARPP32 phosphorylated on threonine 75.
Applicazioni
Anti-phospho-DARPP32 (pThr75) antibody produced in rabbit is suitable for dot blot and western blot analysis at a working dilution of 1:1000 using rat caudate lysates.
Azioni biochim/fisiol
Dopamine D1 receptor stimulation enhances cAMP formation, resulting in the phosphorylation of DARPP32. Phosphorylation of DARPP32 plays an important role in the regulation of dopaminergic neurotransmission. The activity of this protein has an important role in the actions of alcohol, caffeine and Prozac®.
Descrizione del bersaglio
DARPP32 phosphorylated on Thr75 acts as an inhibitor of PKA.
Stato fisico
Solution at 100 μL in 10 mM HEPES, pH 7.5, 150 mM NaCl, 100 μg/mL BSA, and 50% glycerol.
Altre note
The labeling by the antibody to DARPP32 Thr75 is markedly increased by okadaic acid treatment.
Note legali
Prozac is a registered trademark of Eli Lilly and Co.
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Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 2
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, type N95 (US)
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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
A A Fienberg et al.
Science (New York, N.Y.), 281(5378), 838-842 (1998-08-07)
Dopaminergic neurons exert a major modulatory effect on the forebrain. Dopamine and adenosine 3',5'-monophosphate-regulated phosphoprotein (32 kilodaltons) (DARPP-32), which is enriched in all neurons that receive a dopaminergic input, is converted in response to dopamine into a potent protein phosphatase
Maria Lindskog et al.
Nature, 418(6899), 774-778 (2002-08-16)
Caffeine has been imbibed since ancient times in tea and coffee, and more recently in colas. Caffeine owes its psychostimulant action to a blockade of adenosine A(2A) receptors, but little is known about its intracellular mechanism of action. Here we
R E Maldve et al.
Nature neuroscience, 5(7), 641-648 (2002-06-18)
The medium spiny neurons of the nucleus accumbens receive both an excitatory glutamatergic input from forebrain and a dopaminergic input from the ventral tegmental area. This integration point may constitute a locus whereby the N-methyl-D-aspartate (NMDA)-subtype of glutamate receptors promotes
Per Svenningsson et al.
Science (New York, N.Y.), 302(5649), 1412-1415 (2003-11-25)
Three distinct classes of drugs: dopaminergic agonists (such as D-amphetamine), serotonergic agonists (such as LSD), and glutamatergic antagonists (such as PCP) all induce psychotomimetic states in experimental animals that closely resemble schizophrenia symptoms in humans. Here we implicate a common
J A Bibb et al.
Nature, 402(6762), 669-671 (1999-12-22)
The physiological state of the cell is controlled by signal transduction mechanisms which regulate the balance between protein kinase and protein phosphatase activities. Here we report that a single protein can, depending on which particular amino-acid residue is phosphorylated, function
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