Passa al contenuto
Merck
Tutte le immagini(2)

Key Documents

G9920

Sigma-Aldrich

Anti-GABAB Receptor 2

affinity isolated antibody, lyophilized powder

Sinonimo/i:

Anti-γ-aminobutyric acid receptor type B subunit 2, Anti-GABABR2

Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali


About This Item

Numero MDL:
Codice UNSPSC:
12352203
NACRES:
NA.41

Origine biologica

rabbit

Livello qualitativo

Coniugato

unconjugated

Forma dell’anticorpo

affinity isolated antibody

Tipo di anticorpo

primary antibodies

Clone

polyclonal

Forma fisica

lyophilized powder

Reattività contro le specie

rat

tecniche

western blot: 1:200 using rat brain membranes

N° accesso UniProt

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

Descrizione generale

The GABAB receptor (GABABR) is a heteromeric G-protein-coupled receptor. It has two subunits-GABAB1 (γ-aminobutyric acid type B receptor subunit 1) and GABAB2 (γ-aminobutyric acid type B receptor subunit 2). In the hippocampus, GABAB2 is present in dendrites and located in the extra-synaptic membrane of spines and dendritic shafts. This gene is located on rat chromosome 5q.

Immunogeno

peptide CKDPIEDINSPEHIQRRLSL corresponding to residues 875-894 of human GABABR2.

Applicazioni

Anti-GABAB Receptor 2 antibody produced in rabbit is suitable for western blotting at a working dilution of 1:200 using rat brain membranes.
Anti-GABAB Receptor 2 antibody has been used in western blotting.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Azioni biochim/fisiol

The inhibitory neurotransmitter GABA signals through two distinct types of pre- and postsynaptic receptors, GABAA and GABAB. Both GABA receptors can regulate depression of synaptic transmission and be involved in the inhibition controlling neuronal excitability. The GABA transmitter interacts with three types of receptors: the ionotropic receptors GABAA and GABAC and the metabotropic GABAB receptor (GABABR). GABABR is a G-protein coupled receptor that plays a key role in slow synaptic inhibition in the brain and spinal cord. GABABR is a heteromer with two subunits, GABABR1 and GABABR2. The amino acids within the second intracellular domain of GABA(B2) are essential for the coupling of GABA(B) receptor heterodimers to their downstream effector systems. The N-termini on both the GB1 and GB2 receptors are involved in the normal coupling of GABAB receptors to their physiological effectors, G(i) and G-protein-activated potassium channels (GIRKs).

Descrizione del bersaglio

The GABABR2 was shown to be essential for trafficking and G-protein binding, while the GABAB R1 subunit was demonstrated to be important for agonist and antagonist binding. GABABR2 is highly expressed in brain especially at the cerebral cortex, thalamus, hippocampus, and spinal cord and to a lesser extent in testis.

Stato fisico

Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA and 0.05% sodium azide.

Ricostituzione

Reconstitute the lyophilized vial with 50 μL or 200 μL deionized water, depending on package size. Further dilutions should be made using a carrier protein such as BSA (1-3%).

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Non trovi il prodotto giusto?  

Prova il nostro Motore di ricerca dei prodotti.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

Possiedi già questo prodotto?

I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.

Visita l’Archivio dei documenti

M Margeta-Mitrovic et al.
Proceedings of the National Academy of Sciences of the United States of America, 98(25), 14649-14654 (2001-11-29)
Many G protein-coupled receptors (GPCRs) have recently been shown to dimerize, and it was suggested that dimerization may be a prerequisite for G protein coupling. gamma-aminobutyric acid type B (GABA(B)) receptors (GPCRs for GABA, a major inhibitory neurotransmitter in the
Yinghui Guo et al.
Experimental and therapeutic medicine, 9(4), 1448-1454 (2015-03-18)
The aim of the present study was to investigate the genes associated with 'anger-in' (tendency to suppress anger) and 'anger-out' (tendency to express anger through verbal or physical means) emotions in humans. Wistar rats were divided into five groups (n=10/group)
Mathias Boller et al.
Journal of neurophysiology, 89(4), 2035-2045 (2003-04-11)
In mammals, GABA(C) receptors seem to be specifically expressed in the retina and the subcortical visual system, with highest extraretinal expression levels in the superior colliculus (SC). Although its presence in the superficial SC has been demonstrated physiologically, a direct
M Margeta-Mitrovic et al.
Proceedings of the National Academy of Sciences of the United States of America, 98(25), 14643-14648 (2001-11-29)
gamma-aminobutyric acid type B (GABA(B)) receptors, G protein-coupled receptors (GPCRs) for GABA, are obligate heterodimers of two homologous subunits, GB1 and GB2. Typical for family C GPCRs, the N termini of both GB1 and GB2 contain a domain with homology
M J Robbins et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 21(20), 8043-8052 (2001-10-06)
GABA(B) receptors are unique among G-protein-coupled receptors (GPCRs) in their requirement for heterodimerization between two homologous subunits, GABA(B1) and GABA(B2), for functional expression. Whereas GABA(B1) is capable of binding receptor agonists and antagonists, the role of each GABA(B) subunit in

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

Contatta l'Assistenza Tecnica.