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Key Documents

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D1946

Sigma-Aldrich

Diaminofluorescein-FM diacetate

≥98% (HPLC)

Sinonimo/i:

4-Amino-5-(N-methylamin)-3′,6′-bis(acetyloxy), DAF-FM DA

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About This Item

Formula empirica (notazione di Hill):
C25H18F2N2O7
Numero CAS:
254109-22-3
Peso molecolare:
496.42
Numero MDL:
MFCD16872050
Codice UNSPSC:
12352200
ID PubChem:
329798717
NACRES:
NA.77

Livello qualitativo

100

Saggio

≥98% (HPLC)

Forma fisica

film

Condizioni di stoccaggio

desiccated

Colore

colorless to yellow

Fluorescenza

λex 500 nm; λem 515 nm in DMSO

Condizioni di spedizione

wet ice

Temperatura di conservazione

−20°C

Stringa SMILE

CNc1ccc2c(c1N)C(=O)OC23c4cc(F)c(OC(C)=O)cc4Oc5cc(OC(C)=O)c(F)cc35

InChI

1S/C25H18F2N2O7/c1-10(30)33-20-8-18-13(6-15(20)26)25(12-4-5-17(29-3)23(28)22(12)24(32)36-25)14-7-16(27)21(34-11(2)31)9-19(14)35-18/h4-9,29H,28H2,1-3H3
BEVHTVRRVVEMEF-UHFFFAOYSA-N

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Applicazioni

DAF-FM DA has been used to measure the nitric oxide (NO) levels in liver tissue homogenates. It has also been used in NO staining for detection of NO from human umbilical vein endothelial cells (HUVECs).

Azioni biochim/fisiol

DAF-FM DA is a cell-permeable fluorescent probe for the detection of nitric oxide (NO). DAF-2 is the original compound and is widely used, but DAF-FM and DAF-FM DA are more sensitive to nitric oxide (NO) compared with DAF-2 and more photo-stable and less pH sensitive. DAF-FM DA permeates well into living cells and is rapidly transformed into water-soluble DAF-FM by cytosolic esterases.

Caratteristiche e vantaggi

This compound is featured on the Nitric Oxide Synthases page of the Handbook of Receptor Classification and Signal Transduction. To browse other handbook pages, click here.

Avvertenza

Hygroscopic

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

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Zhang J, et al
Journal of Material Chemistry B: Materials for Biology and Medicine, 3(45), 8782-8795 (2015)
You Jin Lim et al.
Food chemistry, 303, 125376-125376 (2019-08-24)
This study investigated the effects of persistent ultraviolet B (UV-B) irradiation on isoflavone accumulation in soybean sprouts. Three malonyl isoflavones were increased by UV-B. Malonylgenistin specifically accumulated upon UV-B exposure, whereas the other isoflavones were significantly increased under both dark
Bin Li et al.
Journal of aging research, 2011, 673185-673185 (2011-05-18)
Calorie restriction (CR) induces a metabolic shift towards mitochondrial respiration; however, molecular mechanisms underlying CR remain unclear. Recent studies suggest that CR-induced mitochondrial activity is associated with nitric oxide (NO) production. To understand the role of mitochondria in CR, we
Shaoting Du et al.
Journal of experimental botany, 67(3), 893-904 (2015-11-27)
CO2 elevation often alters the plant's nitrate reductase (NR) activity, the first enzyme acting in the nitrate assimilation pathway. However, the mechanism underlying this process remains unknown. The association between elevated CO2-induced alterations of NR activity and nitric oxide (NO)
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Articoli

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