CRISPR18V

Lenti CRISPR Universal Non-Target Control#1 Transduction Particles (LV04 vector)

• Codice UNSPSC: 41106609
• NACRES: NA.51

Proprietà

• Forma fisica: liquid
• Livello qualitativo: 200
• Confezionamento: vial of 200 μL
• Concentrazione: (1x10⁶ TU/ml (via p24 titering assay))
• applicazioni: CRISPR
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −70°C

Descrizione

Descrizione generale

The Lenti CRISPR Non-Target Control#1 Transduction Particles (LV04 vector) include a gRNA sequence that does not target known human, mouse and rat genes. The non-target control#1 transduction particles are useful as a negative control in experiments using CRISPR lentiviral clones and for cell types that are not amenable to transfection. The Lenti CRISPR Non-Target Contro#1 usesl a dual-component system consisting of U6-driven guide RNA (non-target#1) and hPGK-driven Puromycin resistance cassette and Blue Fluorescence Protein for fluorophorescence. This control requires Cas9 be delivered by a separate vector.

Lentiviral-based particles permit efficient infection and integration of the construct into differentiated and non-dividing cells, such as neurons and dendritic cells, overcoming low transfection and integration difficulties when using these cell lines. Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging and envelope plasmids.

Applicazioni

Functional Genomics/Screening/Target Validation

Altre note

Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells, respectively.

Note legali

CRISPR Use License Agreement

Lentiviral, WPRE, and Evrogen Licenses

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable