CMC0009

STEADY Electrocompetent Cells

Escherichia coli, rod shaped

• Codice UNSPSC: 12352200

Proprietà

• product name: STEADY Electrocompetent Cells, for plasmid production using unstable DNA
• Origine biologica: Escherichia coli
• Grado: for molecular biology
• Forma fisica: buffered aqueous solution
• Modalità di accrescimento: adherent or suspension
• Morfologia: rod shaped
• tecniche: microbiological culture: suitable
• Trasformazione cellulare: competent cell type: electrocompetent; transformation efficiency: ≥1 x 10¹⁰ cfu/μg
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −70°C

Descrizione

Descrizione generale

The STEADY Chemically Competent Cells are a commonly used strain for cloning sequences that suffer unwanted recombination events in other strains.
Clones with inverted repeats or other sequnces prone to recombination are commonly found in retroviral genes, and require cells such as this product to be propagated stably.

Genotype

recA13 supE44 ara-14 galK2 lacY1 proA2 rpsL20(StrR) xyl-5 λ– leu mtl-1 F– mcrB mrr hsdS20(rB–, mB–)

Caratteristiche e vantaggi

• Clone unstable sequences with reliable results
• Available as chemically competent or electrocompetent cells
• Your choice of two packaging formats
• High efficiency: over 1 × 10⁸ cfu/μg (chem) or 1 × 10¹⁰ cfu/μg (electro)
• Excellent value

Componenti

• STEADY electrocompetent cells
• pUC 19 transformation control DNA
• recovery medium for cloning

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids