CLL1221

Safe Harbor Landing Pad Cell Line Jurkat T-Lymphocytes

human male peripheral blood (Source Disease: Acute T cell leukemia)

• Sinonimo/i: T-Lymphocyte Cell Line
• Codice UNSPSC: 41106514
• NACRES: NA.81

Proprietà

• Nome del prodotto: Safe Harbor Landing Pad Cell Line Jurkat T-Lymphocytes,
• Origine biologica: human male peripheral blood (Source Disease: Acute T cell leukemia)
• Livello qualitativo: 200
• Stato: frozen liquid (Vial of Frozen Cells)
• Modalità di accrescimento: Suspension
• tecniche: cell culture | mammalian: suitable
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Descrizione generale

The STR profile of this cell line matches that of its parental cell line ATCC^® Catalog No. TIB-152. Jurkat T lymphocytes are a human, acute T cell lymphoma cell line isolated in the late 1970s from the peripheral blood of a young male patient suffering from T cell leukemia. The cells possess a pseudodiploid karyotype and have been characterized as expressing CD3 and, upon stimulation, interleukin-2.

Descrizione della linea cellulare

These cells are a human, acute T cell lymphoma cell line isolated from the peripheral blood of a young male suffering from T cell leukemia. The cells possess a pseudodiploid karyotype, express CD3 and, upon stimulation, IL-2.

Applicazioni

This product is a human Jurkat T-lymphocyte cell line in which a landing pad cassette has been integrated into the AAVS1 safe harbor locus using CompoZr^® Zinc Finger Nuclease technology. The mKATE2 fluorescence gene was integrated following the EF1a promoter and flanked by unique Cre-lox sites. The design of this landing pad cassette allows for easy, fast, and affordable genetic modification using Cre recombinase. mKATE2 can easily be exchanged for a payload of the user′s choice using Cre recombinase and a targeting vector with appropriate lox sites. Cells can then be sorted via fluorescence-activated cell sorting (FACS) for loss of mKATE2 expression as a surrogate for successful integration of the targeting vector. Approximately 7-10 days are required for loss of the mKATE2 signal in successfully targeted cells. See technical bulletin for detailed protocols.

Caratteristiche e vantaggi

These cells contain the mKATE2 fluorescence gene flanked by unique Cre-lox sites inserted in the AAVS1 safe harbor gene. These Jurkat cells are grown in suspension with a doubling time of approximately 22 hours.

Qualità

Tested for Mycoplasma, bacterial and fungal content, post-freeze viability, short terminal repeat (STR) analysis for cell line identification.

Terreno di coltura

RPMI modified to contain 2mM L-glutamine, 10mM HEPES, 1mM sodium pyruvate, and 1500mg/L sodium bicarbonate. Add 10% FBS(Catalog Number F2442).

Note legali

ATCC is a registered trademark of American Type Culture Collection

CompoZr is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Pittogrammi: GHS05
• Avvertenze: Warning
• Indicazioni di pericolo: H290
• Consigli di prudenza: P234 - P390
• Classi di pericolo: Met. Corr. 1
• Codice della classe di stoccaggio: 8A - Combustible corrosive hazardous materials
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable