Passa al contenuto
Merck
Tutte le immagini(1)

Key Documents

View All Documentation

CG200

Sigma-Aldrich

cGMP Enzyme Immunoassay Kit, Direct

sufficient for 96 assays

Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
Tutte le immagini(1)

About This Item

Codice UNSPSC:
12161503
NACRES:
NA.84

impiego

sufficient for 96 assays

Livello qualitativo

200

tecniche

ELISA: suitable

Condizioni di spedizione

wet ice

Temperatura di conservazione

−20°C

Informazioni sul gene

human ... PRKG1(5592) , PRKG2(5593)

Applicazioni

Non-radioactive, competitive immunoassay for the quantitation of total cGMP in tissue and cell cultures. Buffer contains 0.1N HCl that aids in the lysis of cells, inhibits endogenous phosphodiesterases and stabilizes the cyclic nucleotides. This kit uses a polyclonal antibody to cGMP to competitively bind cGMP or cGMP that has been covalently linked to an alkaline phosphatase molecule. The assay is performed in a 96 well plate coated with anti-rabbit IgG antibody. The colored end product, produced by the addition of substrate to the wells, is read at 405 nm on a multiwell plate reader. The intensity of the color is inversely proportional to the concentration of cGMP present in the well.
Please refer to the attached Protocolfor details.

Solo come componenti del kit

N° Catalogo
Descrizione
  • 0.1 M Hydrochloride Acid 1 x 30
  • Acetic Anhydride 1 x 1
  • cGMP-Alkaline Phosphatase Conjugate 1 x 5
  • cGMP EIA Antibody Rabbit Anti-cGMP 1 x 5
  • Cyclic GMP Standard 1 x 0.5
  • Goat Anti-Rabbit IgG Coated 96 Well Microtiter Plate 1 ea
  • Neutralizing Reagent 1 x 6
  • p-Nitrophenyl Phosphate Substrate Solution 1 x 20
  • Plate Sealer 1 ea
  • Stop Solution 1 x 5
  • Triethylamine 1 x 2
  • Wash Buffer Concentrate 1 x 30
Vedi tutto (12)

Avvertenze

Danger

Indicazioni di pericolo

H226,H290,H302,H311,H314,H330,H335

Classi di pericolo

Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Corr. 1A - STOT SE 3

Organi bersaglio

Respiratory system

Rischi supp

EUH071

Codice della classe di stoccaggio

3 - Flammable liquids

Punto d’infiammabilità (°F)

120.2 °F

Punto d’infiammabilità (°C)

49 °C

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

Possiedi già questo prodotto?

I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.

Visita l’Archivio dei documenti

Lance M Wheeler et al.
Nature communications, 8(1), 1722-1722 (2017-11-25)
Materials with switchable absorption properties have been widely used for smart window applications to reduce energy consumption and enhance occupant comfort in buildings. In this work, we combine the benefits of smart windows with energy conversion by producing a photovoltaic
Yuichiro Kunai et al.
Journal of the American Chemical Society, 139(43), 15328-15336 (2017-10-08)
The concept of electrical energy generation based on asymmetric chemical doping of single-walled carbon nanotube (SWNT) papers is presented. We explore 27 small, organic, electron-acceptor molecules that are shown to tune the output open-circuit voltage (V
Madiha A H Hassan et al.
BMC pharmacology, 5, 10-10 (2005-04-09)
Cardiac hypertrophy is a major risk factor for morbidity and mortality in a number of cardiovascular diseases. Consequently, the signaling pathways that inhibit cardiac hypertrophy are currently receiving much interest. Among them, nitric oxide (NO), signaling via cGMP and cGMP-dependent
Chard, T.
An Introduction to Radioimmunoassay and Related Techniques (1990)
Tijssen, P.
Practice and Theory of Enzyme Immunoassays (1985)

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

Contatta l'Assistenza Tecnica.