JVM-2

96090515, human unknown/unspecified, Lymphoblastoid

• Codice UNSPSC: 41106514

Proprietà

• product name: JVM-2, 96090515
• Origine biologica: human unknown/unspecified
• Modalità di accrescimento: Suspension
• Cariotipo: 46,XX,-8,+t(3;8)(p12-13;q12),t(11;14)(q13;q32)
• Morfologia: Lymphoblastoid
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Human Caucasian B-prolymphocytic leukaemia, EBV-transformed

Descrizione della linea cellulare

JVM-2 has been derived from EBV and TPA treated mononuclear cells from a 63 year old female patient with B-prolymphocytic leukaemia. Thirty-three percent of cells were shown to be positive for surface lambda light chain, 37% for IgA, 29% for IgG and 3% for IgM and IgD. In addition, 50% were found to express cytoplasmic IgM and lambda light chain. Expression of the following leukocyte differentiation antigens was found: CD9, CD19, CD20, CD23, CD24, MA6, CD37, CD38, FMC1, FMC7, MHC Class I, FMC16, MHC Class II DR/DP/DQ. The immunoglobulin heavy-and light-chain genes showed the same pattern of rearrangement as in the original prolymphocytes. The cells are lymphoblastoid with large floating aggregates present. The cell line has been eradicated from mycoplasma at ECACC.

Applicazioni

Ig expression of neoplastic cells

Profilo DNA

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 11,13
D16S539: 12,13
D5S818: 11,12
D7S820: 10,11
THO1: 6,9
TPOX: 8,11
vWA: 17

Terreno di coltura

RPMI 1640 +2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Maintain cultures between 3-9 x 100,000/ml; 5% CO₂; 37°C.

Altre note

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