OE21 Cell Line human

Caucasian oesophageal squamous cell carcinoma, 96062201

• Sinonimo/i: JROECL 21 Cells, OE 21 Cells
• Codice UNSPSC: 41106514

Proprietà

• Origine biologica: human esophagus
• Modalità di accrescimento: Adherent
• Cariotipo: Aneuploid
• Morfologia: Epithelial
• Prodotti: Not specified
• Recettori: MHC class I
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Human Caucasian oesophageal squamous cell carcinoma

Descrizione della linea cellulare

OE21, also known as JROECL21, was established in 1993 from a squamous carcinoma of mid oesophagus of a 74 year-old male patient. The tumour was identified as pathological stage IIA(UICC) and showed moderate differentiation. HLA-A,-B and -C (MHC class I) are expressed constitutively, expression of ICAM-1 can be induced by treatment with interferon-gamma. OE21 cells express epithelial cytokeratins and are tumourigenic in nude mice.

Applicazioni

Study of oesophageal cancer

Profilo DNA

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 12
D16S539: 13
D5S818: 12,13
D7S820: 11,12
THO1: 8
TPOX: 8,11
vWA: 16,17

Terreno di coltura

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:8 i.e. seeding at 1x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Altre note

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