XrS6-hamKu80

96012301, hamster ovary, Not specified

• Codice UNSPSC: 41106514

Proprietà

• Nome del prodotto: XrS6-hamKu80, 96012301
• Origine biologica: hamster ovary
• Confezionamento: tube of 5 μg 96012301-DNA-5UG; pkg of vial of cells 96012301-1VL
• Modalità di accrescimento: Adherent
• Cariotipo: Not specified
• Morfologia: Not specified
• Prodotti: Ku80
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Hamster Chinese ovary, transformed with hamster Ku80 cDNA

Descrizione della linea cellulare

The cell line Xrs6-hamKu80 was derived from a radiosensitive CHO-K1 mutant transfected with hamster Ku80 cDNA. Ku80, a subunit of the Ku protein and component of the DNA-dependent protein kinase, is a gene product defective in radiosensitive CHO cells, so called xrs mutants. XRC5, the gene encoding Ku80, belongs to the ionisation radiation group affected in DNA repair defects. Mutants transfected with hamster Ku80 obtained full restoration of gamma-ray resistance, DNA end-binding activity to parent levels and expression of Ku80 and Ku70 proteins.

Applicazioni

Study of DNA repair mechanisms

Terreno di coltura

Ham′s F12 + 2mM Glutamine + 300μg/ml G418 + 10% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Altre note

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