GP2d

NOTE: Both the cell line and DNA from the cell line may beavailable for this product. Please choose -1VL or VIAL for cells, or -DNA-5UGfor DNA, 95090714, human colon, Epithelial

• Sinonimo/i: GP2d Cells
• Codice UNSPSC: 41106514

Proprietà

• Origine biologica: human colon
• Confezionamento: tube of 5 μg 95090714-DNA-5UG; pkg of vial of cells 95090714-1VL
• Modalità di accrescimento: Adherent
• Cariotipo: Diploid
• Morfologia: Epithelial
• Prodotti: Not specified
• Recettori: Epidermal growth factor (EGF)
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Human Caucasian colon adenocarcinoma

Descrizione della linea cellulare

GP2d has been established from the same adenocarcinoma as GP5d (Sigma Catalogue number. 95090715). This has been confirmed by STR profiling at ECACC. The cells were derived from a local recurrence of Duke′s grade B, poorly differentiated carcinoma of the colon from a 71 year old female at surgical resection. The patient received no preoperative chemo- or radiotherapy and had a strong family history of colon cancer with two first degree relatives dying of the disease below the age of 55 years. Both clones possess genetic changes consistent with the pattern of tumour progression in colon cancer but are morphologically distinct. An interstitial deletion on chromosome 5 (region 14q to 22q) and an inverted duplication of bands 10q11 and 10q21 has been reported. Both cell lines contain a normal copy of the ki-ras gene and a copy with a transition in codon 12. GP2d cells show growth response to EGF, TGF α or insulin with an increase in cell numbers. Amphiregulin mRNA is abundant in GP2d but almost undetectable in GP5d.

Applicazioni

GP2d has been used to extract genomic DNA for digital PCR (dPCR). It has also been used to extract DNA from the cell with PIK3CA mutation c.3140A>T.

Confezionamento

NOTE: Both the cell line and DNA from the cell line may beavailable for this product. Please choose -1VL or VIAL for cells, or -DNA-5UGfor DNA.

Profilo DNA

STR-PCR Data: Amelogenin: X
CSF1PO: 9,12
D13S317: 8,12
D16S539: 11,13
D5S818: 11,12
D7S820: 9,11
THO1: 7,9.3
TPOX: 8,11
vWA: 17,18

Terreno di coltura

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 5x10,000 to 1x100,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Altre note

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