CCO

95060212, fish ovary, Fibroblast

• Codice UNSPSC: 41106514

Proprietà

• product name: CCO, 95060212
• Origine biologica: fish ovary
• Modalità di accrescimento: Adherent
• Cariotipo: Not specified
• Morfologia: Fibroblast
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Channel Catfish ovary

Descrizione della linea cellulare

CCO was established from the ovaries of a healthy, juvenile channel catfish, Ictalurus punctatus. The cells grow at temperatures from 10°C to 35°C with the optimum at 30°C and with a reported population doubling up to 12 hours. CCO cells have been shown to replicated channel catfish virus but were refractory to 12 other viruses from fish, birds and mammals.

Applicazioni

Isolation of channel catfish virus

Terreno di coltura

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:3 using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 30°C. Fish cell lines detach easily during transit if the culture is too young. After resuscitation split cells 1:3 and culture for at least one week with 1-2 media ch

Altre note

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