H5

94101905, rat liver, Fusiform

• Codice UNSPSC: 41106514

Proprietà

• product name: H5, 94101905
• Origine biologica: rat liver
• Modalità di accrescimento: Adherent
• Cariotipo: Modal no. 52 (50-55)
• Morfologia: Fusiform
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Rat hepatoma

Descrizione della linea cellulare

H5 is a subclone of H4-II-E-C3 (Sigma Catalogue number. 85061112) which originated from the Reuber H-35 hepatoma induced by chemical carcinogenesis in an A x C rat. The dedifferentiated H5 cells express high levels of glucose-6-phosphate dehydrogenase and exhibit a population doubling time of 12 hours. Differentiated variants are H4IIEC3 (85061112), Fao and others.

Applicazioni

Toxicity studies; negative control for differentiated hepatoma

Terreno di coltura

Coon′s modified Ham′s F12 (or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L Inositol) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:50 to 1:100 i.e. seeding at 1-2x1,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Altre note

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.