KYSE-70

94072012, human esophagus, Epithelial

• Sinonimo/i: KY70 Cells, KYSE70 Cells
• Codice UNSPSC: 41106514

Proprietà

• product name: KYSE-70, 94072012
• Origine biologica: human esophagus
• Modalità di accrescimento: Adherent
• Cariotipo: Hypotriploid with 8% polyploidy
• Morfologia: Epithelial
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Human oesophageal squamous cell carcinoma

Descrizione della linea cellulare

Established from a poorly differentiated invasive oesophageal squamous cell carcinoma resected from middle intra-thoracic oesophagus of a 77-year-old Japanese man prior to treatment. This cell line has been mycoplasma eradicated at ECACC. By short tandem repeat (STR)-PCR analysis the Y chromosome could not be detected in this cell line. It is a known phenomenon that SSC cell lines can lose their Y chromosome.

Terreno di coltura

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

Mantenimento delle subcolture

Split confluent cultures 1:5 to 1:10 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Doubling time ~35 hours.

Altre note

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