V79-4

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 93010723, hamster lung, Fibroblast

• Codice UNSPSC: 41106514

Proprietà

• Origine biologica: hamster lung
• Confezionamento: tube of 5 μg 93010723-DNA-5UG; pkg of vial of cells 93010723-1VL
• Modalità di accrescimento: Adherent
• Cariotipo: 2n = 22
• Morfologia: Fibroblast
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Hamster Chinese lung

Descrizione della linea cellulare

Derived from the lung tissue of a male Chinese hamster. The cells exhibit a high plating efficiency and a doubling time of 12-14 hours. Originally used in the discovery of X-ray induced damage and recovery in mammalian cells. This is a clone of cell line V79 (Sigma Catalogue number. 86041102).

Applicazioni

Mutagenesis and mammalian cell genetic studies

Terreno di coltura

DMEM + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:6 to 1:14 i.e. seeding at 3x10,000-1x100,000 cells/cm²; 5% CO₂; 37°C using 0.25% trypsin/EDTA.

Altre note

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.