70Z/3

91092002, mouse blood, Lymphoblastoid

• Codice UNSPSC: 41106514
• Origine biologica: mouse blood
• Modalità di accrescimento: Suspension
• Cariotipo: Not specified
• Morfologia: Lymphoblastoid
• Prodotti: Cytoplasmic Immunoglobulin
• Recettori: Not specified
• Malattie correlate: cancer

Proprietà

• Nome del prodotto: 70Z/3, 91092002
• Origine biologica: mouse blood
• Modalità di accrescimento: Suspension
• Cariotipo: Not specified
• Morfologia: Lymphoblastoid
• Prodotti: Cytoplasmic Immunoglobulin
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Mouse pre-B lymphocyte

Descrizione della linea cellulare

Derived from a methyl nitrosourea-induced tumour in a (C57BL/6XDBA/2) F1 mouse. This line is positive for cytoplasmic Ig and negative for surface membrane Ig and TdT.

Terreno di coltura

RPMI 1640 + 2mM Glutamine + 0.05mM 2-Mercaptoethanol (2ME) + 10% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Maintain cultures between 2-5x100,000 cells/ml; 5% CO₂; 37°C.

Altre note

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Depositor and originator: Dr P Kincade, Oklahoma Medical Research Foundation