GS-109-V-63

90110503, human skin, Fibroblast

• Codice UNSPSC: 41106514

Proprietà

• Nome del prodotto: GS-109-V-63, 90110503
• Origine biologica: human skin
• Confezionamento: tube of 5 μg 90110503-DNA-5UG; pkg of vial of cells 90110503-1VL
• Modalità di accrescimento: Adherent
• Cariotipo: Modal no. 46
• Morfologia: Fibroblast
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Human Caucasian skin fibroblast Gardner′s syndrome

Descrizione della linea cellulare

Derived from a 13 year old Caucasian male with Gardner′s syndrome, an autosomal dominant condition which predisposes to carcinoma and multiple polyps of the colon. The modal chromosome number was 46, occurring in 60% of cells.

Applicazioni

Tumourigenicity studies

Profilo DNA

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11
D13S317: 11,12
D16S539: 12,13
D5S818: 11,12
D7S820: 9,10
THO1: 7,8
TPOX: 8,11
vWA: 14,17

Terreno di coltura

EMEM (HBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 20% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split semi-confluent cultures 1:2 i.e. seeding at 4x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C. Monolayer should form in a 25cm² flask in 3-4 days.

Altre note

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