PC-9 (formerly known as PC-14)

90071810, human lung, Heterogeneous mixture of round cells and spindle shaped cells

• Sinonimo/i: PC-9/S1 Cells, PC-9S1 Cells, PC9 Cells
• Codice UNSPSC: 41106514

Proprietà

• product name: PC-9 (formerly known as PC-14), 90071810
• Origine biologica: human lung
• Modalità di accrescimento: Adherent/suspension
• Cariotipo: Not specified
• Morfologia: Heterogeneous mixture of round cells and spindle shaped cells
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Human adenocarcinoma

Descrizione della linea cellulare

Derived from a human adenocarcinoma from lung tissue which remains undifferentiated.

PC-14 was originally deposited with the Riken BioResource Center as a cell line derived from human lung adenocarcinoma (undifferentiated type) in 1989. The RIKEN BioResource Center subsequently informed the European Collection of Cell Cultures (ECACC) that short tandem repeat (STR) DNA profile analysis, undertaken in collaboration Immuno-Biological Laboratories Co., Ltd Japan, has shown this cell line to be identical to PC-9 a cell line derived from human lung adenocarcinoma (differentiated type). The misidentification occurred prior to the cell line being deposited at the RIKEN BioResource Center. The name of the cell line has been changed to PC-9 to reflect this finding.

Applicazioni

PC-9 cell line has been used to study the effects of α-tocopherol addition on various cytotoxic drugs.

Tumourigenicity studies

Profilo DNA

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 8
D16S539: 9
D5S818: 11
D7S820: 10
THO1: 7
TPOX: 11
vWA: 17

Terreno di coltura

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Seed cells at 2-4 x 10,000 cells/cm²; 5% CO₂; 37°C. Remove attached cells by brief treatment with 0.25% trypsin or trypsin/EDTA.  

Altre note

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