SW 403

87071008, human colon, Epithelial

• Codice UNSPSC: 41106514

Proprietà

• product name: SW 403, 87071008
• Origine biologica: human colon
• Modalità di accrescimento: Adherent
• Cariotipo: 2n = 46, triploid
• Morfologia: Epithelial
• Prodotti: Carcinoembryonic antigen (CEA)
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Human Caucasian colon adenocarcinoma

Descrizione della linea cellulare

Established from a grade III adenocarcinoma of the bowel of a 51 year old Caucasian female. The cells produce CEA and are tumorigenic in nude mice. Electron microscopy reveals prominent golgi apparatus.

Applicazioni

Tumourigenicity studies

Terreno di coltura

L15 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-3x10,000 cells/cm² using 0.25% trypsin/EDTA; No CO₂; 37°C. Cells do not reach confluency but will grow in small islands.

Altre note

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