GH3

87012603, rat brain, Epithelial

• Codice UNSPSC: 41106514

Proprietà

• product name: GH3, 87012603
• Origine biologica: rat brain
• Modalità di accrescimento: Adherent
• Cariotipo: Hyperdiploid, modal no. 60
• Morfologia: Epithelial
• Prodotti: Growth hormone, prolactin
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Rat Wistar-Furth pituitary tumour

Descrizione della linea cellulare

Derived from a 7 month female Wistar-Furth rat. GH3 cells produce growth hormone faster than the GH1 cell line and also produce prolactin. Hydrocortisone stimulates growth hormone production and inhibits prolactin production. Studies have shown that cells are capable of adaptation to suspension culture using MEM.

Applicazioni

Hormone studies

Terreno di coltura

Ham′s F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Upon thawing, the time taken before the first subculture can be performed may be extended (10-15 days). To aid attatchment, flask

Altre note

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