Hs 578T

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 86082104, human breast, Epithelial

• Codice UNSPSC: 41106514

Proprietà

• Origine biologica: human breast
• Confezionamento: tube of 5 μg 86082104-DNA-5UG; pkg of vial of cells 86082104-1VL
• Modalità di accrescimento: Adherent
• Cariotipo: 2n = 46
• Morfologia: Epithelial
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Human breast carcinoma

Descrizione della linea cellulare

Tumorigenic in immunosuppressed mice and form colonies in semisolid medium. Neither oestrogen receptors nor endogenous viruses were detected.

Applicazioni

Hs 578T has been used to measure the calcium-dependent binding of cadherin-11 and N-cadherin. It has also been used to study the changes in gene expression induced by insulin-like growth factor-binding protein (IGFBP-2).

Profilo DNA

STR-PCR Data: Amelogenin: X
CSF1PO: 13
D13S317: 11
D16S539: 9,12
D5S818: 11
D7S820: 10
THO1: 9,9.3
TPOX: 8
vWA: 17

Terreno di coltura

DMEM + 2mM Glutamine + 10μg/ml Bovine Insulin + 10% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Altre note

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