HT115

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 85061104, human colon, Epithelial

• Sinonimo/i: HT-115 Cells
• Codice UNSPSC: 41106514

Proprietà

• Origine biologica: human colon
• Confezionamento: tube of 5 μg 85061104-DNA-5UG; pkg of vial of cells 85061104-1VL
• Modalità di accrescimento: Adherent
• Cariotipo: Not specified
• Morfologia: Epithelial
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Human colon carcinoma

Descrizione della linea cellulare

Cells possess Ca antigen as defined by the Ca1 antibody. The Ca antigen has been shown to be absent in non-malignant human cell strains and is considered to be a marker linked with malignancy. Island cell growth will degenerate if left more than 9 days between passages.

Applicazioni

Tumourigenicity studies

Profilo DNA

STR-PCR Data: Amelogenin: X
CSF1PO: 7,12
D13S317: 13
D16S539: 10
D5S818: 12
D7S820: 11,12
THO1: 6,9
TPOX: 8
vWA: 18

Terreno di coltura

DMEM + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Cells grow relatively slowly, in islands.

Altre note

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