MRC-5 SV1 TG1

85042501, human lung (fetal), Fibroblast

• Codice UNSPSC: 41106514

Proprietà

• product name: MRC-5 SV1 TG1, 85042501
• Origine biologica: human lung (fetal)
• Modalità di accrescimento: Adherent
• Cariotipo: Modal no. = 60-80 hyperdiploid
• Morfologia: Fibroblast
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Human foetal lung, SV40 transformed

Descrizione della linea cellulare

A clonal derivative of an SV40 transformed permanent line of MRC-5. Selection was made using X-ray induction in the presence of 2μg/ml thioguanine. The cells are HAT sensitive.

Terreno di coltura

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 1-2x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Altre note

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