Passa al contenuto
Merck
Tutte le immagini(1)

Key Documents

C-12212

Sigma-Aldrich

Human Dermal Microvascular Endothelial Cells (HDMEC)

Adult, 500,000 cryopreserved cells

Sinonimo/i:

HDMEC, Microvascular Endothelial Cells

Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali


About This Item

Codice UNSPSC:
41106514
NACRES:
NA.81

Origine biologica

human dermis (adult)

Confezionamento

pkg of 500,000 cells

Morfologia

( endothelial)

tecniche

cell culture | mammalian: suitable

Condizioni di spedizione

dry ice

Temperatura di conservazione

−196°C

Descrizione generale

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.

Origine della linea cellulare

Skin

Applicazioni

Primary Human Dermal Microvascular Endothelial Cells (HDMEC) are isolated from the dermis of adult skin (different locations) and are provided in a cryopreserved format. Since the dermis contains blood and lymphatic capillaries, HDMEC comprise Blood and Lymphatic Microvascular Endothelial Cells. Both have a common origin and can be identified by several markers.

Qualità

Rigid quality control tests are performed for each lot of Microvascular Endothelial Cells. They are tested for cell morphology and cell type specific markers, e.g. von Willebrand Factor (vWF), CD31 and Podoplanin using flow cytometric analyses. Growth performance is tested through multiple passages up to 15 population doublings (PD) without antibiotics or antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Attenzione

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Mantenimento delle subcolture

Click here for more information.

Altre note

Recommended Plating Density: 10000 - 20000 cells per cm2Passage After Thawing: P2Tested Markers: von Willebrand Factor (vWF) positive, CD31 positive, Dil-Ac-LDL uptake positiveGuaranteed population doublings: >15

Prodotti consigliati

Recommended Primary Cell Culture Media:Link

Esclusione di responsabilità

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

Possiedi già questo prodotto?

I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.

Visita l’Archivio dei documenti

Protocolli

Cell culture protocol: the endothelial cell transwell migration and invasion assay used to study angiogenesis and cancer cell metastasis. Explore over 350 PromoCell products.

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

Contatta l'Assistenza Tecnica.