3T3 Swiss Albino

85022108, mouse embryo, Fibroblast

• Codice UNSPSC: 41106514

Proprietà

• product name: 3T3 Swiss Albino, 85022108
• Origine biologica: mouse embryo
• Modalità di accrescimento: Adherent
• Cariotipo: 2n = 40; hypertriploid
• Morfologia: Fibroblast
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Descrizione generale

Product has lost contact inhibition

Origine della linea cellulare

Mouse Swiss Albino embryo fibroblast

Descrizione della linea cellulare

Evolved under conditions favouring retention of the property of contact inhibition of cell proliferation; they exhibit much more contact inhibition than diploid fibroblasts freshly plated in culture. Readily transformed by polyoma and SV40 viruses resulting in reduced sensitivity to contact inhibition. Cells have now lost their contact inhibition.

Applicazioni

Contact inhibition and virus studies: polyoma and SV40 viruses

Terreno di coltura

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:10 to 1:20 i.e. seeding at 5x1,000 to 1x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Altre note

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