LIM2551
12070505, human colon, epithelial; forms aggregates of small rounded cells, adherent and suspended.
Sinonimo/i:
LIM 2551, LIM-2551
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About This Item
Codice UNSPSC:
41106514
Origine biologica:
human colon
Modalità di accrescimento:
Semi-adherent
Cariotipo:
Not specified
Morfologia:
Epithelial; grows as loose aggregates of small rounded cells, some adherent, others in suspension.
Recettori:
Not specified
Malattie correlate:
cancer
Prodotti consigliati
Nome del prodotto
LIM2551, 12070505
Origine biologica
human colon
Modalità di accrescimento
Semi-adherent
Cariotipo
Not specified
Morfologia
Epithelial; grows as loose aggregates of small rounded cells, some adherent, others in suspension.
Recettori
Not specified
Malattie correlate
cancer
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Origine della linea cellulare
Human derived from an adenocarcinoma of the transverse colon
Descrizione della linea cellulare
LIM2551 was derived from an adenocarcinoma of the transverse colon. Cell Bank Australia reference number: CBA0170.
Profilo DNA
Not specified
Terreno di coltura
RPMI 1640 (R0883) (with 2 mM L-Glutamine (G7513) + 25mM HEPES) + FBS / FCS (F2442), Insulin 0.6 μg/ml, Hydrocortisone 1 μg/ml, 1-Thioglycerol 10 μM.
Mantenimento delle subcolture
Optimal split ratio 1:8-1:16 (seeding density 1 x 104 cells/cm2). Cells incubated at 37 °C with 5% CO2. Harvest the cells using 0.05% Trypsin/EDTA at 37 °C for 5 min. LIM2551 requires the growth medium to be changed 3 times each week. Feed and passage as for semi-adherent culture (collect medium with floating cells and then trypsinise the adherent population, pool both together before reseeding).
Altre note
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Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
Depositor and originator: Professor Tony Burgess - Ludwig Institute for Cancer Research Ltd, Melbourne, Australia
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