JU77 cell line human

10092309, human lung, epithelioid: spindly with few vacuoles. Cobblestone morphology at confluency.

• Codice UNSPSC: 41106514

Proprietà

• Nome del prodotto: JU77 cell line human, 10092309
• Origine biologica: human lung
• Descrizione: Malignant mesothelioma cells from human lung
• Confezionamento: tube of 5 μg 10092309-DNA-5UG; pkg of vial of cells 10092309-1VL
• Modalità di accrescimento: Adherent
• Cariotipo: Not specified
• Morfologia: Epithelial-like: cells are spindle-shaped with few vacuoles. Upon confluence develop an epithelioid "cobblestone-like mat"
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Human malignant mesothelioma

Descrizione della linea cellulare

This cell line was derived from the pleural fluid of a patient with malignant mesothelioma. The patient had known exposure to crocidolite asbestos. JU77 is not tumourigenic in nude mice. Cells express cytokeratin and epithelial membrane antigen (EMA) but not mucin. Cells are spindle shaped with few vacuoles. Cell Bank Australia reference number: CBA0140

Applicazioni

Investigation of the properties of malignant mesothelioma cells and the development of new diagnostic methods.

Terreno di coltura

RPMI 1640 (with 2mM L-Glutamine+ 25mM HEPES) + 5% FCS

Mantenimento delle subcolture

Optimal split ratio 1:8 (seeding density 0.8 x104 cells/cm²).Cells incubated at 37°C with 5% CO₂.Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 min.

Altre note

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Depositor and originator: Richard Lake - University of Western Australia, Australia