Mero-14

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 9100101, human lung, Spindle shaped cells

• Sinonimo/i: Mero14 Cells
• Codice UNSPSC: 41106514

Proprietà

• Origine biologica: human lung
• Confezionamento: tube of 5 μg 09100101-DNA-5UG; pkg of vial of cells 09100101-1VL
• Modalità di accrescimento: Adherent
• Cariotipo: Number of chromosomes 72-75
• Morfologia: Spindle shaped cells
• Prodotti: Cytokeratins 7, 8, 18 and 19. Epithelial membrane antigen (EMA)
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

A human mesothelioma cell line derived from a malignant mesothelioma. Asbestos exposure

Descrizione della linea cellulare

Mero-14 was derived from the pleural effusion from a male patient aged >60 with a history of asbestos exposure. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.

Applicazioni

Investigation of the properties of malignant mesothelioma cells and the development of new diagnostic methods.

Profilo DNA

STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 12
D16S539: 12,13
D5S818: 9
D7S820: 10,11
THO1: 8
TPOX: 9,11
vWA: 15,18

Terreno di coltura

Hams F10 + 15% FCS + 2mM Glutamine

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Population doubling approx 24hrs

Altre note

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