CAD cell line from mouse

8100805, mouse nerve, Neuronal

• Sinonimo/i: CAD Cell Line
• Codice UNSPSC: 41106514

Proprietà

• Nome del prodotto: CAD cell line from mouse, 08100805
• Origine biologica: mouse nerve
• Descrizione: Mouse (B6/D2 F1 hybrid) catecholaminergic neuronal tumor
• Modalità di accrescimento: Adherent
• Cariotipo: Not specified
• Morfologia: Neuronal
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Mouse (B6/D2 F1 hybrid) catecholaminergic neuronal tumour

Descrizione della linea cellulare

CAD cells (Cath.-a-differentiated) are a variant of a CNS catecholaminergic cell line established from a brain tumour in a transgenic mouse carrying wild-type SV40 T antigen under transcriptional control of rat tyrosine hydroxylase promoter. CAD has lost the immortalising oncogene and expresses neurone-specific proteins and synaptic vesicle proteins. CAD will differentiate to form neuronal processes in serum free conditions.

Applicazioni

Study of neuronal differentiation

Terreno di coltura

DMEM:HAMS F12 (1:1) + 2mM Glutamine + 8% Foetal Bovine Serum (FBS)

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at 3 X 10⁴ cells/cm² Cells should be dislodged by gentle pipetting; 5% CO₂; 37°C.

Altre note

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.