L929/R

4102001, mouse adipose tissue, Fibroblast

• Codice UNSPSC: 41106514

Proprietà

• Nome del prodotto: L929/R, 04102001
• Origine biologica: mouse adipose tissue
• Confezionamento: tube of 5 μg 04102001-DNA-5UG; pkg of vial of cells 04102001-1VL
• Modalità di accrescimento: Adherent
• Cariotipo: Not specified
• Morfologia: Fibroblast
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Mouse C3H/An connective tissue cisplatin resistant

Descrizione della linea cellulare

This cisplatin-resistant cell line has been developed by exposure of the parent L929 murine fibroblast cell line (Sigma Catalogue number. 85011425) to increasing concentrations of cisplatin in vitro. L929/R cells can be used in the development of novel anti-cancer treatments. The parent cell line L929 was derived from normal subcutaneous areolar adipose tissue.

Applicazioni

Development of novel anti-cancer treatments

Terreno di coltura

RPMI 1640 + 2mM L-Glutamine + 10% Foetal Bovine Serum (FBS) + 0.26μM Cisplatin.

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:5 to 1:50 i.e. seeding at 1-2x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 2% CO₂; 37 C.

Altre note

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