RCC4 plus vector alone

3112702, human kidney, Epithelial

• Sinonimo/i: Human RCC4, RCC4 Cell Line
• Codice UNSPSC: 41106514

Proprietà

• Nome del prodotto: RCC4 plus vector alone, 03112702
• Origine biologica: human kidney
• Modalità di accrescimento: Adherent
• Cariotipo: Not specified
• Morfologia: Epithelial
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Renal cell carcinoma cell line RCC4 stably transfected with an empty expression vector, pcDNA3, conferring neomycin resistance.

Descrizione della linea cellulare

Renal cell carcinoma cell line RCC4 stably transfected with an empty expression vector, pcDNA3, conferring neomycin resistance. This cell line is VHL-deficient i.e. the von Hippel-Lindau tumour suppressor gene product is not expressed. These cells are tumourigenic.

Applicazioni

RCC4 plus vector alone is mainly used to study the tumour suppressor gene product function - von Hippel-Lindau tumour suppressor gene. It is also used in cell responses to hypoxia.

• RCC4 plus vector alone has been used in study involving the effect of in vitro ischemia on canonical hypoxic HIF-1α (Hypoxia Inducible Factor α) stabilization.
• It has been used in the study involving CMA (chaperone-mediated autophagy)-mediated degradation of HIF1α in the lysosome.
• It has also been used to study lipodepsipeptide MDN-0066-mediated inhibition of VHL/HIF (Von Hippel-Lindau/hypoxia inducible factor) pathway.

Profilo DNA

STR-PCR Data: Amelogenin: X
CSF1PO: 10,12
D13S317: 12
D16S539: 11
D5S818: 12
D7S820: 8.2
THO1: 9.3
TPOX: 11
vWA: 16

Terreno di coltura

DMEM + 10% FBS + 2 mM Glutamine + G418 0.5mg/ml

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:4 to 1:8 i.e. seeding at 1-3 x 10,000 cells/cm² using 0.25% Trypsin/EDTA; 5% CO₂; 37°C.

Altre note

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