C5233

Carboxypeptidase B from human pancreas

50-55 units/mg protein carboxypeptidase B

• Sinonimo/i: Peptidyl-L-Lysine[L-arginine] hydrolase
• Numero CAS: 9025-24-5
• Classificazione EC (Enzyme Commission): 3.4.17.2 (BRENDA, IUBMB)
• Numero MDL: MFCD00081476
• Codice UNSPSC: 12352204
• ID PubChem: 24892769
• NACRES: NA.54

Proprietà

• Origine biologica: human pancreas
• Livello qualitativo: 200
• Forma fisica: solution
• Attività specifica: 50-55 units/mg protein carboxypeptidase B
• Impurezze: ≤0.2% chymotrypsin; ≤0.2% trypsin; ≤1 unit/mg protein carboxypeptidase A
• N° accesso UniProt: P15086
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −20°C
• InChI: 1S/C31H38N4O7S/c1-20(32-26(36)15-16-27(37)38)28(39)33-21(2)30(41)35-17-9-14-25(35)29(40)34-24(18-22-10-5-3-6-11-22)31(42)43-19-23-12-7-4-8-13-23/h3-8,10-13,20-21,24-25H,9,14-19H2,1-2H3,(H,32,36)(H,33,39)(H,34,40)(H,37,38); TWURVFFNODFJBJ-UHFFFAOYSA-N
• Informazioni sul gene: human ... CPB1(1360)

Descrizione

Descrizione generale

Carboxypeptidase B is mapped to human chromosome 3q24. Carboxypeptidase B belongs to A/B subfamily of carboxypeptidases.

Applicazioni

Carboxypeptidase B from Sigma has been used as a reference for assaying carboxypeptidase activity in lysed pituitary granules derived from the anterior and intermediate lobes of rat. The enzyme has also been used to digest plasma samples by removing C-terminal basic amino acids, to get a distinct band for each allotype during C4 electrophoresis.

Azioni biochim/fisiol

Carboxypeptidase B (or peptidyl-L-lysine (-L-arginine) hydrolase) catalyzes the hydrolysis of the basic amino acids, lysine, arginine, and ornithine from the C-terminal position of polypeptides. It has been shown to be a single polypeptide of 34,000 Da. Trypsin is capable of converting native enzyme to the active enzyme, carboxypeptidase B II in vitro. The optimum pH is found to be 9.0. The enzyme may be used for sequence analysis by successive cleavage of C-terminal basic amino acids. It can also be used as a serum marker for the diagnosis of acute pancreatitis.

Mutations in the carboxypeptidase B (CPB1) gene is implicated with increased susceptibility to pancreatic cancer development and progression. Elevated levels of CPB1 is associated with low grade breast tumors and lymph node positive grade 1 tumors.

Definizione di unità

One unit will hydrolyze 1 μmole of hippuryl-L-arginine per minute at pH 7.7 at 25 °C

Stato fisico

Solution in 0.05 M NaOAc pH 5.0 + 1.0 M NaCl + 0.01% NaN₃

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable