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Merck
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Documenti fondamentali

B3148

Sigma-Aldrich

Anti-Goat/Sheep IgG−Biotin antibody, Mouse monoclonal

clone GT-34, purified from hybridoma cell culture

Sinonimo/i:

Monoclonal Anti-Goat/Sheep IgG, Monoclonal Anti-Goat/Sheep IgG–Biotin antibody produced in mouse

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About This Item

Codice UNSPSC:
12352203
NACRES:
NA.46

Origine biologica

mouse

Livello qualitativo

200

Coniugato

biotin conjugate

Forma dell’anticorpo

purified from hybridoma cell culture

Tipo di anticorpo

secondary antibodies

Clone

GT-34, monoclonal

Forma fisica

buffered aqueous solution

Reattività contro le specie

sheep, goat, bovine

Non deve reagire con

rat, canine, mouse, rabbit, guinea pig, chicken, horse, pig, feline, human

tecniche

direct ELISA: 1:5,000

Isotipo

IgG1

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

modifica post-traduzionali bersaglio

unmodified

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Descrizione generale

Monoclonal Anti-Goat IgG (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.
The antibody reacts with an epitope located on the heavy chain of goat IgG1 and IgG2.
The product binds to all goat/sheep IgG1

Applicazioni

Monoclonal Anti-Goat/Sheep IgG-Biotin antibody produced in mouse has been used in:
  • enzyme linked immuno sorbent assay (ELISA)
  • immunoblotting
  • dot blotting
  • immunohistochemistry
  • immunocytochemistry

Monoclonal Anti-Goat/Sheep IgG-Biotin antibody produced in mouse may be used for ELISA at a minimum working dilution of 1:5000. It was used at a working dilution of 1:1000 to visualize collagen type IV in mouse digit sections.

Azioni biochim/fisiol

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. The coupling of biotin allows for the binding of various labels such as avidin or streptavidin.

Stato fisico

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

nwg

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Dynamic changes after murine digit amputation: the MRL mouse digit shows waves of tissue remodeling, growth, and apoptosis
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Wound Repair and Regeneration, 17(3), 447 -4455 (2009)
S C Jones et al.
Gut, 36(5), 724-730 (1995-05-01)
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L Li et al.
Scandinavian journal of immunology, 57(5), 410-422 (2003-05-20)
Involution of the thymus and alterations in the development of thymocytes are the most prominent features of age-related immune senescence. We have carried out a comparative analysis of thymocyte and stroma in rapid thymic involution DBA/2 (D2) strain of mice
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Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society, 17(3), 447-455 (2009-08-08)
Digit regrowth following amputation injury proximal to the first phalangeal joint is not a property of mammalian wound healing. However, the regenerative potential observed in the MRL mouse invites a reexamination of this rule. In this study, healing was assessed
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