B2904

Bluo-Gal

reagent for selection of recombinant bacterial clones

• Sinonimo/i: 5-Bromo-3-indolyl β-D-galactopyranoside, substrate for β-galactosidase, Bluo-Gal
• Formula empirica (notazione di Hill): C₁₄H₁₆BrNO₆
• Numero CAS: 97753-82-7
• Peso molecolare: 374.18
• Beilstein: 1550140
• Numero MDL: MFCD00063691
• Codice UNSPSC: 12352200
• ID PubChem: 24891417
• NACRES: NA.85

Proprietà

• Grado: for molecular biology
• Livello qualitativo: 200
• Sterilità: non-sterile
• Saggio: ≥98% (HPLC)
• Forma fisica: powder
• tecniche: nucleic acid detection: suitable
• Solubilità: DMF: soluble
• Compatibilità: suitable for substrate for β-galactosidase
• Temperatura di conservazione: −20°C
• Stringa SMILE: OC[C@H]1O[C@@H](Oc2c[nH]c3ccc(Br)cc23)[C@H](O)[C@@H](O)[C@H]1O
• InChI: 1S/C14H16BrNO6/c15-6-1-2-8-7(3-6)9(4-16-8)21-14-13(20)12(19)11(18)10(5-17)22-14/h1-4,10-14,16-20H,5H2/t10-,11+,12+,13-,14-/m1/s1; LINMATFDVHBYOS-MBJXGIAVSA-N

Descrizione

Descrizione generale

Bluo-Gal is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. Bluo-Gal is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.

Applicazioni

Bluo-Gal is suitable for use in selection of recombinant bacterial colonies with the lac⁺ phenotype. Bluo-Gal is suitable for use as β-galactosidase substrate in the staining of skin tissues for electron microscopy analysis, Dictyostelium samples and in the staining of vena cava of adult mice.

Chromogenic substrate suitable for identification of lac⁺ bacterial colonies. An alternative to 5-bromo-4-chloro-3-indolyl β-D-galactopyranoside (X-Gal), producing a darker blue color.

Principio

When Bluo-Gal is hydrolyzed by β-galactosidase, the resulting product will form a blue precipitate. Lac+ colonies grown in the presence of Bluo-Gal turn a deeper blue color more intense than X-Gal, allowing for easy differentiation between lac+ and lac- colonies.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, type N95 (US)