XGAL-RO

X-Gal

solubility: in DMF, soluble, solubility: in methanol, soluble, storage temp.:2-8°C

• Sinonimo/i: 5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside, 5-bromo-4-chloro-3-indolyl-β-d-galactopyranoside, bromo-4-chloro-3-indolyl-β-d-galactopyranoside, 5-, 5-Bromo-4-chloro-3-indolyl β-D-galactoside, BCIG, X-Gal
• Formula empirica (notazione di Hill): C₁₄H₁₅BrClNO₆
• Numero CAS: 7240-90-6
• Peso molecolare: 408.63
• Beilstein: 1402009
• Numero MDL: MFCD00005666
• Codice UNSPSC: 12352204
• ID PubChem: 329830810
• NACRES: NA.28

Proprietà

• Descrizione: 5-Bromo-4-chloro-3-indolyl-β-D-galactopyranoside
• Livello qualitativo: 100
• Saggio: 99%
• Forma fisica: solid
• PM: 408.63 g/mol
• Confezionamento: pkg of 1 g (10745740001); pkg of 100 mg (03117073001); pkg of 100 mg (11680293001); pkg of 2.5 g (10703729001); pkg of 250 mg (10651745001)
• Produttore/marchio commerciale: Roche
• Concentrazione: 20 mg/mL
• tecniche: activity assay: suitable
• Colore: white
• Solubilità: DMF: soluble; methanol: soluble
• applicazioni: genomic analysis; life science and biopharma
• Temperatura di conservazione: 2-8°C
• Stringa SMILE: OC[C@H]1O[C@@H](Oc2c[nH]c3ccc(Br)c(Cl)c23)[C@H](O)[C@@H](O)[C@H]1O
• InChI: 1S/C14H15BrClNO6/c15-5-1-2-6-9(10(5)16)7(3-17-6)22-14-13(21)12(20)11(19)8(4-18)23-14/h1-3,8,11-14,17-21H,4H2/t8-,11+,12+,13-,14-/m1/s1; OPIFSICVWOWJMJ-AEOCFKNESA-N

Descrizione

Descrizione generale

X-Gal is a chromogenic substrate for β-galactosidase that produces a rich blue color that can easily be detected visually over background. It is soluble in dimethylformamide and methanol. The product is not soluble in water.

Applicazioni

X-Gal has been used for immunohistocytochemistry applications. It is also the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac⁺ genotype.

X-Gal is a chromogenic substrate for β-galactosidase that produces a rich blue color that can easily be detected visually over background. X-Gal is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac⁺ genotype.

Qualità

Chromatographically homogeneous

Stato fisico

Crystals, white powder

Nota sulla preparazione

Working solution: Stock solution:

• 20 mg/ml in dimethyl formamide (DMF), clear, colorless solution
• A_{590 nm}: 0.01

Storage conditions (working solution): X-gal in solution (i.e., dissolved in DMF 2% (w/v)) is stable for several weeks when stored in the dark at 2 to 8 °C; when the solution is stored at -15 to -25 °C, it is stable for one year.

Altre note

For life science research only. Not for use in diagnostic procedures.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable