83330

Pyruvate Kinase from rabbit muscle

crystalline suspension in 3.8 M ammonium sulfate solution, ~350 U/mg protein (10-40 mg/ml)

• Sinonimo/i: ATP:pyruvate 2-O-phosphotransferase, PK
• Numero CAS: 9001-59-6
• Classificazione EC (Enzyme Commission): 2.7.1.40 (BRENDA, IUBMB)
• Numero CE: 232-616-2
• Numero MDL: MFCD00081946
• Codice UNSPSC: 12352204
• eCl@ss: 32160410
• NACRES: NA.54

Proprietà

• Stato: suspension
• Qualità: crystalline suspension in 3.8 M ammonium sulfate solution
• Attività specifica: ~350 U/mg protein (10-40 mg/ml)
• PM: M_r ~230000
• pH: ~6
• Attività estranea: lactate dehydrogenase ≤0.01%
• Temperatura di conservazione: 2-8°C

Descrizione

Azioni biochim/fisiol

Molecular Weight: 237 kDa and exists as a tetramer of four equal subunits of molecular weight 57 kDa.
Isoelectric Point: 7.6
Optimal pH: ∼7.5
Optimal Temperature: 25°C
ΕA₂₈₀ = 0.54 for 1 mg(p)/ml, 1 cm path
Reported K_M values are ATP (0.86 mM), pyruvate (10 mM), ADP (0.3 mM), and PEP (0.07 mM) in Tris buffer at pH 7.4 and 30 °C. Pyruvate kinase is highly specific for phosphoenolpyruvate, but can utilize other dinucleotide triphosphates as substrates in place of ATP including GTP, ITP, dATP, UTP, and CTP.

Pyruvate kinase catalyzes the conversion of phosphoenolpyruvate to pyruvate, whitch is the rate-limiting final step in glycolysis.

Definizione di unità

1 U corresponds to the amount of enzyme which catalyzes the transphosphorylation of 1 μmol PEP per min at pH 7.4 and 25 °C

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, type N95 (US)