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75633

Sigma-Aldrich

Osmium tetroxide solution

suitable for electron microscopy, 2% in H2O

Sinonimo/i:

Osmium(IV) oxide

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About This Item

Formula condensata:
OsO4
Numero CAS:
20816-12-0
Peso molecolare:
254.23
Numero MDL:
MFCD00011150
Codice UNSPSC:
12171500
ID PubChem:
57652577
NACRES:
NA.47

Grado

for analytical purposes

Stato

solution

Impiego in reazioni chimiche

reagent type: oxidant

Concentrazione

2% in H2O

tecniche

electron microscopy: suitable

Colore

colorless to yellow

Densità

1.0 g/mL at 20 °C
1.0 g/mL at 20 °C

applicazioni

hematology
histology

Temperatura di conservazione

2-8°C

Stringa SMILE

O=[Os](=O)(=O)=O

InChI

1S/4O.Os
VUVGYHUDAICLFK-UHFFFAOYSA-N

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Descrizione generale

Osmium tetroxide (OsO4) also referred to as osmium, is a heavy tetrahedral, symmetrical molecule. It is both a dye and a non-coagulant fixative agent in electron microscopy primarily used for the examination of biological tissue components in the histopathology laboratory. Specimens are postfixed with osmium tetroxide to ensure the preservation of the lipids.

Applicazioni

Osmium tetroxide solution has been used as a fixative in electron microscopy.

Azioni biochim/fisiol

Osmium tetroxide is also referred to as osmium and is mainly used as a secondary fixative of tissues. The molecule reacts with lipid moieties and is responsible for the oxidation of unsaturated bonds of fatty acids. This reaction adds density and contrast to biological samples. In normal tissues, presence of osmium results in intense black staining which can be viewed by normal eye. In addition, it can be used as a mordant (a molecule which combines with stain or dye at later stages) in electron microscopy, thereby enhancing lead staining.

Pittogrammi

Skull and crossbonesCorrosion
GHS06,GHS05

Avvertenze

Danger

Indicazioni di pericolo

H302 + H332,H310,H315,H318

Classi di pericolo

Acute Tox. 2 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Skin Irrit. 2

Codice della classe di stoccaggio

6.1B - Non-combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

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Certificati d'analisi (COA)

Lot/Batch Number
BCCK2370
BCCH6755
BCCF5871
BCCC6174
BCCB9800

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