55212
Phalloidin–Atto Rho6G
suitable for fluorescence, ≥90% (HPCE)
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About This Item
Codice UNSPSC:
12352200
NACRES:
NA.32
Prodotti consigliati
Livello qualitativo
Saggio
≥90% (HPCE)
Forma fisica
solid
PM
Mw 1396 g/mol
Produttore/marchio commerciale
ATTO-TEC GmbH
Fluorescenza
λex 535 nm; λem 560 nm in 0.1 M phosphate pH 7.0
Compatibilità
suitable for fluorescence
Temperatura di conservazione
−20°C
Descrizione generale
Atto Rho6G is a novel fluorescent label that belongs to the class of Rhodamine dyes. It shows a strong absorption, extraordinary high fluorescence quantum yield, high thermal and photostability, and a very little triplet formation. Atto Rho6G is moderately hydrophilic.Phalloidin is a fungal toxin isolated from the poisonous mushroom Amanita phalloides. Its toxicity is attributed to the ability to bind F-actin in liver and muscle cells. As a result of binding phalloidin, actin filaments become strongly stabilized. Phalloidin has been found to bind only to polymeric and oligomeric forms of actin, and not to monomeric actin. The dissociation constant of the actin-phalloidin complex has been determined to be on the order of 3 x 10–8. Phalloidin differs from amanitin in rapidity of action; at high dose levels, death of mice or rats occurs within 1 or 2 hours.
Applicazioni
Fluorescent conjugates of phalloidin, rhodamine-phalloidin staining reagents, such as Phalloidin–Atto Rho6G are used to label actin filaments for histological applications. Some structural features of phalloidin are required for the binding to actin. However, the side chain of amino acid 7 (g-dihydroxyleucine) is accessible for chemical modifications without appreciable loss of affinity for actin.
Note legali
This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.
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Molecular brain, 6, 42-42 (2013-10-08)
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Quantum dots (QDs) are fluorescent nanocrystals whose unique properties are fundamentally different from organic fluorophores. Moreover, their cores display sufficient electron density to be visible under transmission electron microscopy (TEM). Here, we report a technique for phalloidin-based TEM detection of
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