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Merck
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Documenti fondamentali

51290

Sigma-Aldrich

Hemoglobin from bovine blood

lyophilized, 2×cryst., ≥90% (SDS-PAGE), powder

Sinonimo/i:

Bovine hemoglobin, Hb, Methemoglobin

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About This Item

Numero CAS:
9008-02-0
Numero MDL:
MFCD00131282
Codice UNSPSC:
12352202

Origine biologica

bovine blood

Livello qualitativo

100

Saggio

≥90% (SDS-PAGE)

Forma fisica

powder

Qualità

2×cryst.
lyophilized

PM

Mr ~64500

tecniche

UV/Vis spectroscopy: suitable

Impurezze

salt, none detected
~0.3% Fe

Perdita

≤8% loss on drying

N° accesso UniProt

P01966
P02070
P02081
P06642
P06643

Temperatura di conservazione

2-8°C

Informazioni sul gene

cow ... HBA1(281221) , HBB(280813) , HBE2(513240) , HBE4(513108) , HBG(511735) , alpha globin(512439)

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Descrizione generale

Hemoglobin (Hb) is the protein responsible for storage and transport of oxygen and other gaseous ligands in red blood cells (RBC). Hb is also the pioneer material for synthesis of Hb-based oxygen carriers (HBOCs) that can be used as substitutes for RBC.

Applicazioni

The solubility of α-elastin has been applied to construction of elastin-mimetic biomaterials.

Azioni biochim/fisiol

Oxygen transporter. The Fe2+/Fe3+ balance is a physiological indicator of blood oxygenation; deoxygenated hemoglobin accessorizes a feedback loop by reducing nitrite to NO, a vasodilator which enhances blood flow to oxygen-deprived tissues.

Avvertenza

Since native hemoglobin is readily oxidized in air, these preparations may be predominantly methemoglobin.

Altre note

Review: Oxygen carrier proteins
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Applicazioni

N° Catalogo
Descrizione
Determinazione del prezzo

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

Certificati d'analisi (COA)

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Paul W Buehler et al.
Biomaterials, 31(13), 3723-3735 (2010-02-13)
Hemoglobin-based oxygen carriers (HBOC) are currently being developed as red blood cell (RBC) substitutes for use in transfusion medicine. Despite significant commercial development, late stage clinical results of polymerized hemoglobin (PolyHb) solutions hamper development. We synthesized two types of PolyHbs
M. Brunori et al.
Topics in Molecular and Structural Biology, 7, 263-263 (1985)
R Bokiniec et al.
Advances in medical sciences, 57(2), 348-355 (2012-11-20)
The aim of this study was to determine brain oxygenation in full-term and preterm neonates using near infrared spectroscopy. A total of 88 full-term and preterm newborn infants without hypoxic-ischaemic disorders admitted to the NICU were examined using NIRS on
Jae G Kim et al.
Journal of biomedical optics, 17(10), 105005-105005 (2012-12-12)
Noninvasive near infrared spectroscopy measurements were performed to monitor cyanide (CN) poisoning and recovery in the brain region and in foreleg muscle simultaneously, and the effects of a novel CN antidote, sulfanegen sodium, on tissue hemoglobin oxygenation changes were compared
Enrico Bucci et al.
Biochemistry, 52(24), 4149-4156 (2013-05-29)
Hill's plots of oxygen binding isotherms reveal the presence of a transition between two different oxygen affinities at the beginning and end of the isotherm. They correspond to the two conformations anticipated by the MWC model, namely, the T and
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Chromatograms

application for HPLC

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