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Key Documents

402OA-05A

Sigma-Aldrich

Human Chondrocytes - Osteoarthritis: HC-OA, adult

Sinonimo/i:

HC-OA cells

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About This Item

Codice UNSPSC:
41106514
NACRES:
NA.81

Origine biologica

human articular cartilage (osteoarthritic)

Livello qualitativo

Confezionamento

pkg of 500,000 cells

Produttore/marchio commerciale

Cell Applications, Inc

Modalità di accrescimento

Adherent

Cariotipo

2n = 46

Morfologia

chondrocyte

tecniche

cell culture | mammalian: suitable

Malattie correlate

arthritis

Condizioni di spedizione

dry ice

Temperatura di conservazione

−196°C

Descrizione generale

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.

HC-OA Human Chondrocytes are derived from human articular cartilage of donors with osteoarthritis (OA). Osteoarthritis is an inflammatory disease characterized by increased degradation of cartilage tissue in the joint due to overproduction of enzymes degrading the extracellular matrix. Despite the initial proliferation and activation of chondrocytes, they are not able to efficiently repair the degrading cartilage. Instead, chondrocytes undergo terminal differentiation and eventually apoptose, leading to mineralization of cartilage in a process resembling bone formation during development. Thus, HC-OA provide a useful model to study changes in chondrocyte biology in response to abnormal environment of the OA joint.

Characterization: Positive for aggrecan after differentiation.

HC-OA have been used to demonstrate that THF-alpha activates transcription of ADAMTS-4, a metalloproteinase implicated in cartilage degradation in OA, via p38-MAPK-dependent mechanism (Xue, 2013).

Origine della linea cellulare

Cartilage

Applicazioni

model for studying chondrocyte biology in an osteoarthritis joint

Componenti

Basal Medium containing 10% FBS & 10% DMSO

Nota sulla preparazione

  • 1st passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
  • Can be cultured at least 10 doublings

Mantenimento delle subcolture

Please refer to the HC-OA Culture Protocol.

Esclusione di responsabilità

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

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Hongfei Zhang et al.
Clinical rheumatology, 39(11), 3473-3478 (2020-05-11)
LncRNA CTBP1-AS2 has been reported to be involved in type 2 diabetes and cardiomyocyte hypertrophy, while its roles in other human diseases are unknown. Our preliminary deep sequencing analysis showed altered expression of CTBP1-AS2 in osteoarthritis (OA). In addition, CTBP1-AS2
Xiangxiang Sun et al.
International journal of biological sciences, 16(6), 994-1009 (2020-03-07)
Osteoarthritis (OA) is a common type of arthritis. Chronic inflammation is an important contributor to the pathogenesis of OA. The maturation and secretion of proinflammatory cytokines are controlled by inflammasomes, especially NLRP1 (NLR Family Pyrin Domain Containing 1) and NLRP3.

Protocolli

Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.

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