10021101

SerBob cell line human

• Codice UNSPSC: 41106514

Proprietà

• Origine biologica: human prostate
• Stato: liquid
• Confezionamento: tube of 5 μg 10021101-DNA-5UG; pkg of vial of cells 10021101-1VL
• Modalità di accrescimento: Adherent
• Cariotipo: 4n (67 to 88)
• Morfologia: Cobblestone
• Prodotti: More differentiated than Bob with increased CD24, PAP, PSCA expression (prostate differentiation markers). 10% 3-D sphere formation in Matrigel. Migratory and invasive when compared to benign prostate tumour. More invasive than Bob.
• Recettori: CD44, alpha2 integrin, alpha6 integrin, EGFR, FGFR1, LIFR
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Human prostate cancer spontaneously immortalized

Descrizione della linea cellulare

First spontaneously immortalized prostate cancer cell line to be established from a trans-rectal needle biopsy (TRBP) of a patient with castration-resistant prostate cancer (CRPC). Bob is a novel pre-clinical model for functional studies in CRPC and especially for studying the CRPC "basal" phenotype. SerBob grows as a monolayer culture with limited differentiation. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.

Applicazioni

Prostate cancer research including drug testing.

Confezionamento

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

Profilo DNA

STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 9,14
D16S539: 10,12
D5S818: 13
D7S820: 10,11
THO1: 9
TPOX: 8,10
vWA: 17

Terreno di coltura

Keratinocyte-SFM (Invitrogen, catalogue no 37010-022, supplied with prequalified human recombinant epidermal growth factor 1-53 (EGF 1-53), bovine pituitary extract (BPE) and glutamine) + 2ng/ml leukaemia inhibitory factor + 2ng/ml stem cell factor + 100ng/ml cholera toxin + 1ng/ml granulocyte macrophage colony stimulating factor.

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:5 to 1:20 i.e. seeding at 2-4 x 104 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. At confluence 5-7 x 104 cells/cm² can be expected.

Altre note

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Esclusione di responsabilità

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable