00062107

MDCK-II Cell Line canine

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• Sinonimo/i: MDCK Type II Cells, MDCK-2 Cells, MDCKII-WT Cells
• Codice UNSPSC: 41106514
• NACRES: NA.81

Proprietà

• Origine biologica: canine kidney
• Livello qualitativo: 100
• Descrizione: Canine Cocker Spaniel Kidney
• Stato: liquid
• Confezionamento: tube of 5 μg 00062107-DNA-5UG; pkg of vial of cells 00062107-1VL
• Modalità di accrescimento: Adherent
• Cariotipo: Dog
• Morfologia: Polarised epithelial
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable

Descrizione

Origine della linea cellulare

Canine Cocker Spaniel Kidney

Descrizione della linea cellulare

MDCK II - Madin-Darby canine kidney- is a subclone derived from the heterogenous parent line - MDCK (Sigma catalogue no. 85011435) MDCK II cells, which predominate in later passages from MDCK, are reported to display electrical resistance of 100 ohm/cm². This strain is thought to be derived from the distal tubule or collecting duct of the nephron. The cell line can be used as an experimental model to study the generation and maintenance of cell surface polarity in epithelial cells. Mycoplasma eradicated using Boehringer method prior to deposit at ECACC.

Confezionamento

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

Terreno di coltura

EMEM (EBSS) (M2279) + 2mM L-Glutamine (G7513) + 5% FBS (F2442)

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:5 twice a week i.e. seeding at 5 x 10,000 cells/cm² using 0.25% tryspin or trypsin/EDTA; 5% CO₂ 37°C,

Altre note

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information. Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable