T1438
Todd Hewitt broth
suitable for microbiology, NutriSelect® Plus
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About This Item
Codice UNSPSC:
41171607
NACRES:
NA.85
Prodotti consigliati
Sterilità
non-sterile
Livello qualitativo
Forma fisica
powder
Durata
limited shelf life, expiry date on the label
Produttore/marchio commerciale
NutriSelect® Plus
tecniche
microbiological culture: suitable
pH
7.8±0.2 (25 °C)
applicazioni
clinical testing
food and beverages
microbiology
Temperatura di conservazione
2-25°C
Compatibilità
nonselective for Streptococcus spp.
Descrizione generale
Todd Hewitt Broth is used for cultivation of haemolytic streptococci for different serological tests. This medium has been recommended as an alternative type in epidemiologic studies of group A streptococci as well as pathogenic microorganisms. Todd Hewitt Broth medium is very nutritive by the presence of peptic digest of animal tissue and beef heart infusion. Dextrose stimulates haemolysin production. The medium is well buffered by sodium phosphate and sodium carbonate to neutralize the acid produced during dextrose fermentation. This restricts destruction of antigenic streptococcal haemolysin. It is also found that sodium phosphate has a stimulating effect on the pneumococcal growth. Todd Hewitt Broth can be employed as an alternative to serum broth or horse flesh digest broth for the cultivation of streptococci prior to serological typing.
Applicazioni
Recommended for the cultivation of group A hemolytic Streptococci used for serological studies.
Todd Hewitt broth has been used for the culture of various microorganisms, such as Bacillus subtilis and Streptococcus pneumoniae.
Componenti
Components (g/L):
Beef Heart Infusion 500.00
Peptic Digest of Animal Tissue 20.00
Dextrose 2.00
Sodium Chloride 2.00
Sodium Phosphate 0.40
Sodium Carbonate 2.50
Beef Heart Infusion 500.00
Peptic Digest of Animal Tissue 20.00
Dextrose 2.00
Sodium Chloride 2.00
Sodium Phosphate 0.40
Sodium Carbonate 2.50
Nota sulla preparazione
Suspend 37 g of Todd Hewitt Broth in 1000 ml of distilled water. Mix well and dispense as desired. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Nota a piè di pagina
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
Note legali
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Faceshields, Gloves, type N95 (US)
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Moody, M.D., et al.
American Journal of Public Health, 53, 1083-1083 (1963)
Todd, E.W., et al.
The Journal of Pathology and Bacteriology, 35, 1-97 (1932)
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Wenzel M, et al.
Bio-protocol (2018)
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Articoli
Streptococci- Overview of Detection, Identification, Differentiation and Cultivation Techniques
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