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Key Documents

B8647

Sigma-Aldrich

Brilliant Blue R Concentrate

methanol solution

Sinonimo/i:

Brilliant Blue R, Acid Blue 83, Brilliant indocyanin 6B, Coomassie Brilliant Blue R

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About This Item

Formula empirica (notazione di Hill):
C45H44N3NaO7S2
Numero CAS:
Peso molecolare:
825.97
Colour Index:
42660
Beilstein:
5718025
Numero MDL:
Codice UNSPSC:
12171500
ID PubChem:
NACRES:
NA.47

product name

Brilliant Blue R Concentrate, suitable for SDS-PAGE, methanol solution

Forma fisica

methanol solution

Livello qualitativo

Colore

dark blue

Compatibilità

suitable for SDS-PAGE

applicazioni

diagnostic assay manufacturing
hematology
histology

Temperatura di conservazione

room temp

Stringa SMILE

[Na+].CCOc1ccc(Nc2ccc(cc2)C(\c3ccc(cc3)N(CC)Cc4cccc(c4)S([O-])(=O)=O)=C5\C=C/C(C=C5)=[N+](\CC)Cc6cccc(c6)S([O-])(=O)=O)cc1

InChI

1S/C45H45N3O7S2.Na/c1-4-47(31-33-9-7-11-43(29-33)56(49,50)51)40-23-15-36(16-24-40)45(35-13-19-38(20-14-35)46-39-21-27-42(28-22-39)55-6-3)37-17-25-41(26-18-37)48(5-2)32-34-10-8-12-44(30-34)57(52,53)54;/h7-30H,4-6,31-32H2,1-3H3,(H2,49,50,51,52,53,54);/q;+1/p-1
NKLPQNGYXWVELD-UHFFFAOYSA-M

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Applicazioni

Brilliant Blue R concentrate is a methanol based stain especially designed for staining protein in polyacrylamide but will also stain agarose gels. The stain contains methanol and acetic acid so gels do not require fixing prior to staining.
Brilliant Blue R Concentrate has been used for protein band detection after nondenaturing gradient gel electrophoresis, isoelectric focussing and SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis).
For detection of protein bands following electrophoresis.

Ricostituzione

After dilution with deionized water to a final volume of one liter, solution will contain 0.25% (w/v) Brilliant Blue R, 40% (v/v) methanol and 7% (v/v) acetic acid.

Note legali

Prodotti correlati

N° Catalogo
Descrizione
Determinazione del prezzo

Avvertenze

Danger

Classi di pericolo

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Eye Irrit. 2 - Flam. Liq. 2 - Skin Irrit. 2 - STOT SE 1

Organi bersaglio

Eyes,Central nervous system

Codice della classe di stoccaggio

3 - Flammable liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

57.2 °F - closed cup

Punto d’infiammabilità (°C)

14 °C - closed cup

Dispositivi di protezione individuale

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter


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The effect of ethanol or hepatotoxin exposure on rat transferrin desialylation.
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Alcohol and Alcoholism (Oxford, Oxfordshire), 31, 445-451 (1996)
Crystallization of soluble proteins in vapor diffusion for x-ray crystallography.
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In sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) according to the method of Weber and Osborn, protein bands are often distorted by tailing at both ends, and the phenomenon is often called "edge tailing." This was eliminated by adding glycerol at

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