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Merck
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Documenti fondamentali

63011

Millipore

M9 Broth

suitable for microbiology, NutriSelect® Basic

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About This Item

Codice UNSPSC:
41171607
NACRES:
NA.74

Sterilità

non-sterile

Livello qualitativo

Forma fisica

powder

Composizione

ammonium chloride, 1 g/L
disodium hydrogen phosphate, 6 g/L
potassium dihydrogen phosphate, 3 g/L
sodium chloride, 0.5 g/L

Produttore/marchio commerciale

NutriSelect® Basic

tecniche

microbiological culture: suitable

pH finale

7.4±0.2 (25 °C)

applicazioni

food and beverages
life science and biopharma

microbiology

Compatibilità

nonselective for Escherichia coli
nonselective for coliforms

Applicazioni

M9 Broth is used as a microbiological growth media and for the preparation of phage and plasmid DNA using E. coli K12 strains, acc. to Miller (1987).

Nota sulla preparazione

Dissolve 10.5 g in 1 litre distilled water and adjust the pH to 7.4. Sterilize by autoclaving at 121°C for 15 minutes. Add 2 ml 1 M magnesium sulfate (Cat. No. 63133; filter sterilized) and 20 ml of 20% solution from a carbon source (such as glucose; Cat. No. 49140)

Nota a piè di pagina

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Note legali

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)


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A bacterial strain isolated from soil for its potential to control the anthracnose disease caused by Colletotrichum gloeosporioides was identified as a Bacillus subtilis. Bacillus subtilis CMB32 produced antifungal agents on M9 broth at 30degreesC. Biosurfactant lipopeptides produced by Bacillus
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Practical aspects of preparing phage and plasmid DNA: growth, maintenance, and storage of bacteria and bacteriophage.
H Miller
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